Three-Day Sickness (Bovine Ephemeral Fever) in Cattle

- Laboratory Diagnosis

Samples

·       Blood during febrile stage

·       Serum samples

·       Tissue samples

Diagnostic Tests

1. RT-PCR

Most sensitive method for viral detection.

2. Virus Isolation

Performed in Cell culture - Suckling mice

3. Serological Tests

·       ELISA

·       Virus neutralization test

·       Complement fixation test

4. Hematology: may show:

·       Leukopenia

·       Neutrophilia

·       Hypocalcemia