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sample collection and sample processing

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"last update: 25 Sep. 2024"                                                                                                        Download Guideline

- Samples in relation to suspected diagnosis

- When submitting samples to a diagnostic laboratory, it is important to provide thorough and relevant flock information on the laboratory submission form.

-Send the swabs to the diagnostic laboratory for immediate analysis.

-Do not freeze samples.

Pathogen

Sample Pooling for PCR

Media Used

Site of the samples

Avian Influenza

pool of 10 swabs

in 5.5mL media pool 5 swabs in 3mL media BHI (Brain heart infusion)

-Tracheal

 -oropharyngeal

Newcastle Disease

pool of 5 swabs

in 5.5mL media BHI

- Tracheal

 -oropharyngeal

Mycoplasma gallisepticum/synoviae

pool of 5 swabs

or BHI media

-Dry Tracheal

-oropharyngeal

Bacteria and/or Viruses only pool tissues from a single bird

pool by organ system (respiratory, enteric, reproductive)

media provided in the culture tube

-Affected organs