- When submitting samples to a diagnostic laboratory, it is important to provide thorough and relevant flock information on the laboratory submission form.
-Send the swabs to the diagnostic laboratory for immediate analysis.
-Do not freeze samples.
|
Pathogen |
Sample Pooling for PCR |
Media Used |
Site of the samples |
|
Avian Influenza |
pool of 10 swabs |
in 5.5mL media pool 5 swabs in 3mL media BHI (Brain heart infusion) |
-Tracheal -oropharyngeal |
|
Newcastle Disease |
pool of 5 swabs |
in 5.5mL media BHI |
- Tracheal -oropharyngeal |
|
Mycoplasma gallisepticum/synoviae |
pool of 5 swabs |
or BHI media |
-Dry Tracheal -oropharyngeal |
|
Bacteria and/or Viruses only pool tissues from a single bird |
pool by organ system (respiratory, enteric, reproductive) |
media provided in the culture tube |
-Affected organs |